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Key chondrocytes were cultured from the existence or absence of IL-1β (10 ng/mL) for 24 h. RNA was collected as stated previously mentioned. Samples had been geared up In accordance with library package maker’s protocol, indexed, pooled, and sequenced on an Illumina HiSeq. Basecalls and demultiplexing were being carried out with Illumina’s

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The site is secure. The https:// guarantees you are connecting to your official Web-site and that any facts you offer is encrypted and transmitted securely.The hiPSC line was derived within the Wellcome Rely on Sanger Institute and has become published18. The usage of this cell line model was authorised by Proportionate Evaluate Subcommittee with t

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